Use este identificador para citar ou linkar para este item: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/571981
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dc.contributor.authorMARTINS, C. F.pt_BR
dc.contributor.authorDODE, M. N.pt_BR
dc.contributor.authorBÁO, S. N.pt_BR
dc.contributor.authorRUMPF, R.pt_BR
dc.date.accessioned2011-07-08T11:54:26Z-
dc.date.available2011-07-08T11:54:26Z-
dc.date.created2008-04-18pt_BR
dc.date.issued2007pt_BR
dc.identifier.citationPlanaltina, DF: Embrapa Cerrados, 2007.pt_BR
dc.identifier.urihttp://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/571981pt_BR
dc.descriptionABSTRACT: The success of the assisted reproductive techniques (ART) in both humans and domestic animals depends of the semen quality. Since there is no test that alone can predict sperm fertility, as more sperm parameters are analyzed during evaluation, more acurated will the result. The evaluation of the DNA integrity during the exam of bovine fresh and frozen sperm is of fundamental importance to avoid embryonic losses. The terminal deoxynucleotidyl transferase mediated dUTP nick end labeling assay (TUNEL) has been an efficient method to detect sperm DNA fragmentation, avoiding problems of embryonic development and decrease of the animal reproductive efficiency. This work presents and describes with the TUNEL technique (principles, advantages and detailed protocol), indicating this tool to evaluate the bovine sperm DNA.pt_BR
dc.language.isoporpt_BR
dc.relation.ispartofseries(Embrapa Cerrados. Documentos, 192).pt_BR
dc.rightsopenAccesspt_BR
dc.subjectBovinaept_BR
dc.titleMétodo de TÚNEL: uma ferramenta alternativa para avaliar a integridade do DNA de espermatozóides bovinos.pt_BR
dc.typeFolhetospt_BR
dc.date.updated2011-07-08T11:54:26Zpt_BR
dc.subject.thesagroBovinopt_BR
dc.subject.thesagroDNApt_BR
dc.subject.thesagroEspermatozóidept_BR
dc.subject.thesagroGenética Animalpt_BR
dc.subject.thesagroReprodução Animalpt_BR
dc.subject.nalthesaurusgeneticspt_BR
dc.subject.nalthesaurusreproductionpt_BR
dc.subject.nalthesaurusspermatozoapt_BR
dc.format.extent226 p.pt_BR
riaa.ainfo.id571981pt_BR
riaa.ainfo.lastupdate2009-10-20pt_BR
dc.contributor.institutionCarlos Frederico Martins, CPAC; Margot Nunes Dode; Sônia Nair Báo; Rodolfo Rumpf.pt_BR
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